A Mediated BOD Biosensor Based on Immobilized B. Subtilis on Three-Dimensional Porous Graphene-Polypyrrole Composite
نویسندگان
چکیده
We have developed a novel mediated biochemical oxygen demand (BOD) biosensor based on immobilized Bacillus subtilis (B. subtilis) on three-dimensional (3D) porous graphene-polypyrrole (rGO-PPy) composite. The 3D porous rGO-PPy composite was prepared using hydrothermal method following with electropolymerization. Then the 3D porous rGO-PPy composite was used as a support for immobilizing negatively charged B. subtilis denoted as rGO-PPy-B through coordination and electrostatic interaction. Further, the prepared rGO-PPy-B was used as a microbial biofilm for establishing a mediated BOD biosensor with ferricyanide as an electronic acceptor. The indirect determination of BOD was performed by electrochemical measuring ferrocyanide generated from a reduced ferricyanide mediator using interdigited ultramicroelectrode array (IUDA) as the working electrode. The experimental results suggested a good linear relationship between the amperometric responses and BOD standard concentrations from 4 to 60 mg/L, with a limit detection of 1.8 mg/L (S/N ≥ 3). The electrochemical measurement of real water samples showed a good agreement with the conventional BOD₅ method, and the good anti-interference as well as the long-term stability were well demonstrated, indicating that the proposed mediated BOD biosensor in this study holds a potential practical application of real water monitoring.
منابع مشابه
Design and Fabrication of Biosensor Based on Immobilized AchE on Modified Electrode by Graphene-multiwall Carbon Nanotubs/Beta Cyclodexterin-chitosan
Organophosphorus (OP) forms an important class of toxic compounds. They inhibit acetyl cholinesterase (AChE, EC 3.1.1.7) that results in respiratory and myocardial malfunctions. Pesticides could be accumulated in vegetables and fruits, so detection of them is very important. The goals of this study are decreasing detection time and detection limit of methyl parathion bioprobe. In this research ...
متن کاملModified Glass Carbon Electrode (GCE) Electropolymerized Polypyrrole Nanofibers with Hemoglobin (Hb) Film as a Unique Biosensor for Nitrite Determination
Abstract: In this study, we were investigated behavior the electrochemical reductionof nitrite at a hemoglobin (Hb) immobilized on glass carbon electrode (GCE) containingpolypyrol nanofiber (ppy) films. Polypyrrole (PPy) nanofibers have been constructed onGCE applying electrochemical technique, and can to deposit diverse polymers onminiaturized electrodes with this commo...
متن کاملAn Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H₂O₂ at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10-6 - 8.5×10-5 M and ...
متن کاملElectrically and Electrochemically Active Composite Based on Polyester/Reduced Graphene Oxide/Polypyrrole with Remarkable Washing Durability
متن کامل
A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol
In the present work, we demonstrate the fabrication of laccase biosensor to detect the catechol (CC) using laccase immobilized on graphene-cellulose microfibers (GR-CMF) composite modified screen printed carbon electrode (SPCE). The direct electrochemical behavior of laccase was investigated using laccase immobilized different modified SPCEs, such as GR/SPCE, CMF/SPCE and GR-CMF/SPCE. Compared ...
متن کامل